Medical Lab Equipment

Current Interests

  • Health Policy
  • Ophthalmology
  • Public Health and Prevention
  • Artificial Intelligence

Research Projects

  • Exploring the History of Eyecare Financing and Delivery in Canada

    Project Supervision: Dr. Raisa Deber PhD, Institute of Health Policy, Management and Evaluation at the University of Toronto

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Clinical and Surgical Management of Vitreoretinal Disease in Adult and Paediatric Populations

    Project Supervision: Dr. Peter J. Kertes MD & Dr. Rajeev H. Muni MD MSc, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Mentor: Dr. Marko M. Popovic MD MPH(C)

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Keratoconus: An Inflammatory Corneal Ectasia?

    Project Supervision: Dr. Clara C. Chan MD and Dr. Neera Singal MD, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Mentors: Dr. Tanya Trinh MBBS and Dr. Michael Mimouni MD

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Patterns in Vision Insurance Coverage, Eye Care Provider Use, and Visual Impairment Amongst Canadians

    Project Supervision: Dr. Ya-Ping Jin MD PhD, Department of Ophthalmology & Vision Sciences at the University of Toronto

    FundingCanadian Research Data Centre Network (CRDCN) from the Social Science and Humanities Research Council (SSHRC), the Canadian Institute for Health Research (CIHR), the Canadian Foundation for Innovation (CFI), and Statistics Canada

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Development of an Online Low Vision Rehabilitation Compendium

    Project Supervision: Dr. Samuel N. Markowitz, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Optimal Conditions for Temporary Storage of Ischemically-Injured and Reperfused Kidney Grafts for Transplantation

    Project Supervision: Dr. Peter Urbanellis MD MSc, UHN Multi-Organ Transplant Program

    Funding: N/A

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Expression of Stem Cell Marker LIFR-α: Understanding its Effect on Differentiation in Human Granulosa Cells Among Women Suffering from Infertility

    Supervising Professor and Principal Investigator: Dr. Shlomit Kenigsberg PhD MBA and Dr. Clifford L. Librach MD FRCSC FACOG(REI), CReATe Fertility Centre

    Funding: Sunnybrook Research Institute Davis + Henderson Student Research Award & CReATe Fertility Centre

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Promoting Competency-Based Surgical Education for Ophthalmology Trainees

    Project Supervision: Dr. Amandeep S. Rai, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Clinical Findings in Patients Presenting to a Tertiary Neuro-Ophthalmology Practice

    Project Supervision: Dr. Jonathan A. Micieli MD, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Quantification of Photic Phenomena in Pseudophakic Multifocal IOL Patients to Develop a Predictive Machine-Learning Algorithm and Enhance Surgical Outcomes: The Halomtr Study

    Project Supervision: Dr. Devesh K. Varma, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Funding: Alcon Canada, Inc., A Novartis Division

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Efficacy and Safety of Novel Microinvasive Glaucoma Surgical Procedures

    Project Supervision: Dr. Matthew B. Schlenker MD MSC and Dr. Iqbal Ike K. Ahmed MD, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Mentor: Dr. Marko M. Popovic MD MPH(C)

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Ameliorating The Provision of Healthcare in Canada

    Project Supervision: Dr. Sherif R. El-Defrawy MD PhD, Department of Ophthalmology & Vision Sciences at the University of Toronto

    Mentors: John Yip MBA ICD.D

    Funding: None.

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Relationships, Community Involvement, and Access to Care Amongst Racial and Gender Minority Groups

    There is currently little knowledge of the experiences and service needs of the Lesbian, Gay, Bisexual, Trans, and Queer (LGBTQ+) community internationally. Research indicates that LGBTQ+ communities are often at greater risk of violence and discrimination, which may also lead to greater mental health problems, suicidal ideation, internalized homophobia, and abuse resulting from the use of online dating applications. Due to this, they often have greater needs for safe services, community support, and protection against this higher risk of harm. This project includes data from the MSc Thesis Data of Raymond M. McKie and data from the OutLook Study.

    Project Supervision: Dr. Robert N. Travers PhD, Department of Health Sciences, Wilfrid Laurier University

    Mentor: Dr. Raymond M. McKie MSc PhD

    Funding: Faculty of Science Students’ Association (FOSSA)

    Associated Publications:

    • You may view all of Prem’s publications here.
  • Expression and Purification of a Recombinant Version of atToc159MHis for Structural and Functional Studies: Understanding the Role of its M-domain

    The M-Domain of TOC complexes, specifically TOC159M, remains a mystery to this day. Understanding how this protein can be expressed in various organisms including that of Escherichia coli is consequential in understanding how TOC159M, a membrane protein, interacts with bacterial membranes. According to Zoonens and Miroux, the so-called internal membrane is also produced when auto-inducing a culture of BL-21 Codon Plus (RIPL) E.coli. The main goal of Prem’s research project is to further understand the expression of protein in membranes, the role of the internal membranes and how they are produced, and analyze the M-domain to determine structure and function. Various methods include auto-induction, IMAC purification, in addition to assays including Bradford, NADH oxidase, BCA, and lipid to determine solubility of the protein in different detergents with an end goal of determining the structure and function of the atTOC159 M-domain.

    Project Supervision: Dr. Matthew D. Smith PhD, Department of Biology, Wilfrid Laurier University

    Mentor: Dr. Tuan (Patrick) Hoang MD PhD MSc

    Funding: Natural Sciences and Engineering Research Council of Canada (NSERC) Undergraduate Student Research Award (USRA) & NSERC Research Discovery Grant

    Associated Publications:

    • You may view all of Prem’s publications here.

Skills

  • Agarose Gel Electrophoresis
  • Aseptic Technique and Autoclaving
  • Analytical Chemistry Techniques
  • Auto-Induction & Bacterial Cell Culture
  • Bacterial Transformation
  • Bradford & Bicinchoninic Acid (BCA) Protein Assays
  • cDNA Synthesis & Pre-Amplification
  • Centrifugation & Fractionation
  • Circular Dichroism
  • Distillation
  • DNA Cloning, Ligation, & Transformation
  • Double Enzyme Restriction Digest
  • Gel DNA Extraction & Purification
  • Immobilized Metal Ion Affiinty Chromatography (IMAC)
  • Immunocytochemistry
  • Liquid-Liquid Separation
  • Plasmid DNA Isolation & Purification
  • Polyacrylamide Gel Electrophoresis
  • Protein Expression & Purification
  • qPCR & Primer Design
  • RNA Extraction
  • Scientific Writing
  • Western Blot Analysis

Laboratory Training, Certifications, and Continuing Education

  • Jan 2021
    Translating Critical Appraisal of a Manuscript into Meaningful Peer Review Certificate, Cochrane Eyes & Vision Group
  • March 2020
    CITI Privacy and Good Clinical Practice
  • August 2019
    WHMIS Safety Training
  • August 2019
    Canada's National Hand Hygiene Challenge
    Canadian Patient Safety Institute - Hand Hygiene
  • August 2015
    EMD Serono ABC's of ART

    A Basic Competency Course of Assisted Reproductive Technologies

    August 26, 2015

    • Module 1: Reproductive Anatomy, Certificate Number 72
    • Module 2: Overview of Infertility, Certificate Number 71
    • Module 3: Infertility Screening, Certificate Number 70
    • Module 4: Introduction to ART, Certificate Number 69
    • Module 5: Medications Used, Certificate Number 68

  • February 2015
    Ontario Worker Health and Safety Awareness in 4 Steps
    Worker Health and Safety Awareness in 4 Steps Certificate
  • September 2014
    Ethical Conduct for Research Involving Humans Course on Research Ethics (TCPS 2: CORE)

    TCPS 2

  • July 2011
    Accessibility Standards Training Program Completion Certificate

    Accessibility Standards Training Program